Analysis configuration file for gene fusion analysis

An analysis configuration file is available to use to set individual assay-specific thresholds for these six properties, as applicable for the type of that individual assay:

  • Minimum read count

  • Minimum normalized read count

  • Minimum wild type ratio

  • Make calls

  • Do not report

  • Maximum read count negative

  • Minimum Imbalance Score, for targets of type RNAExon Tile

  • Minimum Imbalance p-value, for targets of type RNAExon Tile

  • Minumum average read count for Exon Tiling QC, for targets of type RNAExon Tile

The analysis configuration file is a readable and editable tab-separated file that is specific to a fusion panel. For a given panel, the file contains all of the targets in the panel as different rows and has eight columns. The first two columns are the name and type of the target in that row. The assay name is the unique identifier and provides mapping between the analysis configuration file and the reference files that are used by the analysis. You can use the other columns to set the six different properties at individual target level.