Visualize MSI analysis results

  1. There are two ways to visualize MSI analysis results in Ion Reporter™ Software:

    Option

    Description

    Visualize MSI results from one or more analyses simultaneously from the Analyses table.

    In the Analyses table, select a row for an analysis or select the checkbox next to one or more analyses that you want to visualize simultaneously, then click Visualize. Alternatively, click Actions > Visualize.

    Visualize analysis results individually from the Analysis Results screen.

    In the Analyses table, click an analysis hyperlink in the Analysis column to open the Analysis Results, then click Visualize.

    The Analysis Visualization screen opens to the TMB tab.

  2. Click the MSI tab.

    Item

    Description

    MSI Status

    A sample is assigned an MSI status that is based on the MSI Score. The MSI status can be one of the following:

    • MSI-High

    • MSI-Low

    • MSS (MSI stable)

    • No Call (MSI status is intermediate, i.e., between the thresholds)

    • QC Fail

    MSI parameter settings include thresholds that determine whether the status is MSI-High, MSI-Low, or MSS. A status of No Call or QC Fail is based on the MSI marker coverage.

    MSI Score

    A sample-level MSI score that is calculated with individual MSI marker scores. The overall score is used to determine the MSI status of the sample.

    MSI Coverage

    A combined sample-level coverage that is calculated with the individual MSI marker-level coverage.

    A table with MSI Status, MSI Score, and MSI Coverage is shown.

  3. Click Download Results to download a report of the MSI results. The report is downloaded to the folder that is used for downloads, depending on the browser settings.