Read number impact on calculation sensitivity
At above 150,000 mapped reads, the analysis workflow is very sensitive.
Between 20,000 and 150,000 mapped reads, the more reads, the more sensitive the analysis workflow is.
With 20,000 or few mapped reads, we recommend that you rerun the sequencing experiment, if possible. At around 20,000 or fewer reads, the potential for false negatives (missing a real fusion) increases.