IGH V-gene usage and evenness
This is an example of an immunoglobulin heavy chain (IGH) V-gene usage and evenness spectratyping plot from an Oncomine™ BCR IGH‑LR Assay analysis. Circle color indicates the evenness of clone sizes for clones having a particular variable gene-CDR3 nucleotide length combination. Values range from 0 to 1, with 1 indicating most even clone sizes. In this representation, portions of the repertoire containing highly expanded clones appear white. You can further partition the clones by isotype in Ion Reporter™ Software, with the buttons that are above the interactive spectratyping plot.