Visualize metagenomics analysis results with Krona charts

Use the Krona visualization feature to browse through your data using interactive zoomable pie charts. Krona documentation is available at github.

Note: The Snapshot button does not work on the Krona visualization page in the Metagenomics 16S analysis workflow. Krona documentation states that their charts are best viewed with the Firefox™ browser.

  1. In the Analyses tab, click Overview.

  2. In the Analyses screen, click the name of your metagenomics analysis. You can filter the list to display only metagenomics analyses.

  3. In the results screen for the selected analysis, in the Visualization/Downloads section, click the link next to the Krona piechart label.

  4. For the next steps, see the following table or Krona documentation on their github site.

This table describes ways to visualize metagenomics analysis results using Krona charts.

Task

Your action

Open your analysis data in a Krona chart

Click the Visualize results by primer link.

Open Krona on your consensus data

Click the Visualize consensus results link.

Display percentages for one area of the chart

Click that area of the chart. The percentages for that area are displayed in the top right of the page.

Make that area the new focus of the chart

Click the expand box in the top right of the page.

Return to the previous chart focus

Click the back arrow near the Krona logo in the top left of the page. (Only one go-back is supported at a time.)

Change the number of circles shown in the chart

Click the minus or plus icons near the Max depth label.

Change the font size

Click the minus or plus icons near the Font size label.

Change the size of the chart

Click the minus or plus icons near the Chart size label.

Show read depth by color

Select the Color by Reads checkbox. A color legend appears in the bottom left. (This example shows a consensus chart.)

Simplify wedges

Select the Collapse checkbox. This selection combines redundant wedges (that contain only another wedge).

Create an image of the pie chart

Click Snapshot.

Clicking this button creates a SVG (Scalable Vector Graphics) format image file. See Krona documentation on their github site for browser support. (For example, on Chrome™ an SVG file is not created directly. You have to save the page as a PDF file.)

Create a sharable link to the chart

Click Link.

Open Krona documentation

Click the question mark button. This link opens the Krona sourceforge documentation in a new tab.