Analysis workflow templates for Ion AmpliSeq™ HD

Ion Reporter™ Software 5.10 or later contains predefined analysis workflow templates for use with Ion AmpliSeq™ HD panels. Identify the template that best matches your application, then use it to create a custom analysis workflow. For more information, see Create a custom analysis workflow for use with Ion AmpliSeq HD panels.

IMPORTANT! Ensure that the analysis workflow template name that you use matches the type of Ion AmpliSeq™ HD panel files that you include in your analysis. For example, if you use panel files for a liquid biopsy fusions single sample, copy the Ion AmpliSeq™ HD for Liquid Biopsy - w2.2 - Fusions - Single Sample analysis workflow template to create the custom analysis workflow.

Use the following guidelines to find Ion AmpliSeq™ HD analysis analysis workflow templates or analysis workflows that have been copied in the Workflows tab.

  • To filter for Ion AmpliSeq™ HD analysis workflow templates, or copy and edited Ion AmpliSeq™ HD analysis workflows that include saved panel files, search for Target: AmpliSeq HD.

  • To filter for Tumor Ion AmpliSeq™ HD analysis workflow templates, use a combination of the Workflow filters Research Category: Oncology – Solid Tumor, and Target: AmpliSeq HD.

Analysis workflow template

Description

Ion AmpliSeq™ HD for Tumor - w2.2 - DNA - Single Sample

Detects and annotates low frequency (to 0.5% limit of detection) variants (SNPs, INDELs and CNVs) from targeted DNA libraries using Ion AmpliSeq™ HD technology. Compatible with DNA that is purified from tumor samples.

Workflow 2.2 is released with Ion Reporter™ Software 5.12.

Ion AmpliSeq™ HD for Tumor - w2.2 - Fusions - Single Sample

Detects and annotates gene fusions from targeted RNA libraries using Ion AmpliSeq™ HD technology. Compatible with RNA that is purified from tumor samples.

Workflow 2.2 is released with Ion Reporter™ Software 5.12.

Ion AmpliSeq™ HD for Tumor - w2.2 - DNA and Fusions (Single Library) - Single Sample

Detects and annotates low frequency (to 0.5% limit of detection) variants (SNPs, INDELs, CNVs and fusions) from targeted nucleic acid libraries using Ion AmpliSeq™ HD technology. Compatible with DNA and RNA that is purified together from tumor samples.

Workflow 2.2 is released with Ion Reporter™ Software 5.12.

Ion AmpliSeq™ HD for Tumor - w2.2 - DNA and Fusions (Separate Libraries) - Single Sample

Detects and annotates low frequency (to 0.5% limit of detection) somatic variants (SNPs, INDELs and CNVs) from targeted DNA libraries, as well as gene fusions from matching targeted RNA libraries using Ion AmpliSeq™ HD technology. Compatible with DNA and RNA that is purified separately from tumor samples.

Workflow 2.2 is released with Ion Reporter™ Software 5.12.

Ion AmpliSeq™ HD for Liquid Biopsy - w2.2 - DNA - Single Sample

Detects and annotates low frequency (to 0.1% limit of detection) variants (SNPs, INDELs and CNVs) from targeted DNA libraries using Ion AmpliSeq™ HD technology. Compatible with DNA that is purified from cell-free liquid biopsy samples.

Workflow 2.2 is released with Ion Reporter™ Software 5.12.

Ion AmpliSeq™ HD for Liquid Biopsy - w2.2 - Fusions - Single Sample

Detects and annotates gene fusions from targeted RNA libraries using Ion AmpliSeq™ HD technology. Compatible with RNA that is purified from cell-free liquid biopsy samples.

Workflow 2.2 is released with Ion Reporter™ Software 5.12.

Ion AmpliSeq™ HD for Liquid Biopsy - w2.2 - DNA and Fusions (Single Library) - Single Sample

Detects and annotates low frequency (to 0.1% limit of detection) variants (SNPs, INDELs, CNVs and fusions) from targeted nucleic acid libraries using Ion AmpliSeq™ HD technology. Compatible with DNA and RNA that is purified together from cell-free liquid biopsy samples.

Workflow 2.2 is released with Ion Reporter™ Software 5.12.

Ion AmpliSeq™ HD for Liquid Biopsy - w2.2 - DNA and Fusions (Separate Libraries) - Single Sample

Detects and annotates low frequency (to 0.1% limit of detection) somatic variants (SNPs, INDELs and CNVs) from targeted DNA libraries, and gene fusions from matching targeted RNA libraries using Ion AmpliSeq™ HD technology. Compatible with DNA and RNA that is purified separately from cell-free liquid biopsy samples.

Workflow 2.2 is released with Ion Reporter™ Software 5.12.