Somatic hypermutation profile

Immune Repertoire Analysis Results report the frequency of somatic hypermutation (SHM) over the variable gene portion of each clone of a given isotype. This view is available only for the Oncomine™ BCR IGH‑LR Assay and Oncomine™ BCR IGH‑SR Assay. Select SHM profile from the Views list to see a histogram that represents the data.

SHM is calculated by determining the number of mismatches between a clone variable gene sequence and the best matching IMGT® variable gene allele.

Variable gene somatic hypermutation (SHM) by isotype

Click the isotype above the SHM profile plot to further partition the data by isotype.