Molecular Coverage Analysis metrics

Molecular Coverage Analysis metrics are included in a Quality Control report if the molecular Coverage Analysis plugin was run in Torrent Suite™ Software, before data was transferred to and analyzed in Ion Reporter™ Software. These metrics report molecular coverage over targeted regions of the reference genome.

Note: To download a report that contains the metrics, click the Download link in the Molecular coverage analysis report column.

Molecular Coverage analysis metric

Description

Sample Name

The name of the sample, as it was entered into the Planned Run for Torrent Suite™ Software that is used to generate the BAM file that was uploaded to Ion Reporter™ Software.

Barcode

The name of the barcode, as it was entered into the Planed Run for Torrent Suite™ Software that is used to generate the BAM file that was uploaded to Ion Reporter™ Software.

Molecular Uniformity

Percentage of amplicons having molecular coverage between 0.5x and 2x of the median molecular coverage.

Median Reads per Functional Molecule

For each amplicon, the number of reads supporting each functional molecule is averaged across all functional molecules for that amplicon to determine the number of reads per functional molecule at the amplicon level. The median is calculated across all amplicons.

Median Perc Functional Reads

For each amplicon, the percentage of reads supporting functional molecules is (Number of Reads Supporting Functional Molecules) / (Number of Reads). The median is calculated across all amplicons.

Median Molecular Coverage

The number of molecules that satisfies size criteria in the parameters file. The median is calculated across all amplicons.